Journal: Cell

Article Title: Dysregulated Microbial Fermentation of Soluble Fiber Induces Cholestatic Liver Cancer

doi: 10.1016/j.cell.2018.09.004

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Glycodeoxycholic Acid , Cayman Chemical , Item #: 20274.

Techniques: Recombinant, In Situ, Staining, AST Assay, Enzyme-linked Immunosorbent Assay, Cholesterol Assay, Sequencing, Double Knockout, Software

Journal: Frontiers in Microbiology

Article Title: Bile-Salt-Hydrolases from the Probiotic Strain Lactobacillus johnsonii La1 Mediate Anti-giardial Activity in Vitro and in Vivo

doi: 10.3389/fmicb.2017.02707

Figure Lengend Snippet: Substrate specificities of L. johnsonii La1 BSHs. (A) Hydrolase activity of rBSH47 produced in E. coli tested on LB plates. E. coli SE1 wild type or E. coli SE1 expressing rBSH47 were plated in the presence of taurodeoxycholic acid 0.3% (TDCA) or glycodeoxycholic acid 0.3% (GDCA) and incubated for 48–72 h. Activity is detected when a whitish halo forms around colonies. Data with E. coli SE1 expressing rBSH12 and rBSH56, respectively, are not shown. (B) Phylogenetic relationship among selected BSH sequences and substrate prediction. Programme MEGA5 was used for the phylogenetic tree and for the 500-replication bootstrap analysis. The following predicted amino acid sequences were obtained from UniProtKB/Swiss-Prot databases: BSH12, L. johnsonii La1 (Q74IV4_LACJO); BSH47, L. johnsonii La1 (Q74JG0_LACJO); BSH12, L. johnsonii La1 (Q74LX7_LACJO); CBAH-1, C. perfringens strain 13 (P54965.3); BSHA, L. acidophilus LA4 (ACL98173.1); BSHB, L. acidophilus LA4 (ACL98173.1); BSHA, L. acidophilus LA11 (ACL98175.1); BSHB, L. acidophilus LA11 (ACL98176.1); BSHA, L. acidophilus NCFM (YP_193782.1); BSHB, L. acidophilus NCFM (AAV42923.1); BSH , L. gasseri AM1 (ACL98172.1); BSH-α, L. johnsonii 100-100 (AAG22541.1); BSH-β, L. johnsonii 100-100 (AAC34381.1); BSHA, L. johnsonii pf01 (EGP12224.1); BSHB, L. johnsonii pf01 (EGP13287.1); BSHC, L. johnsonii pf01 (EGP12391.1); pCBH1, L. plantarum 80 (AAB24746.1); BSH1, L. plantarum WCSF (CCC80500.1); BlBSH, B. longum subsp. longum (2HF0); BSH, L. acidophilus PF01 (ABQ01980.1); BSH, L. plantarum CK 102 (Ha et al., ); Penicillin V Acylase (PVA), Bacillus sphaericus (3PVA). Substrate specificity, when known (see text for referenced literature) is indicated for each BSH enzyme (in brackets): (TC), specificity for tauro-conjugated bile salts; (GC), specificity for glyco-conjugated bile salts; (TC/GC), specificity for both tauro and glyco-conjugated bile salts.

Article Snippet: Overnight cultures were then spotted on LB agar plates supplemented with either 0.5% taurodeoxycholic acid (TDCA, Sigma-Aldrich) or 0.5% glycodeoxycholic acid (GDCA, Merck Millipore) and incubated at 37°C for 48 h. The BSH hydrolyzing activities were also monitored using purified recombinant enzymes in presence of conjugated bile salts, in solution, by measuring the liberation of amino acids (glycine or taurine) as previously described (Grill et al., ).

Techniques: Activity Assay, Produced, Expressing, Incubation

Journal: Foods

Article Title: The Effects of Enteral Nutrition on the Intestinal Environment in Patients in a Persistent Vegetative State

doi: 10.3390/foods11040549

Figure Lengend Snippet: Metabolomic analysis of intestinal metabolites (nmol/g).

Article Snippet: Pyruvic acid, 2-methylvaleric acid, 4-methylvaleric acid, caproic acid, tauroursodeoxycholic acid (TUDCA), glycoursodeoxycholic acid (GUDCA), ursodeoxycholic acid (UDCA), glycochenodeoxycholic acid (GCDCA), glycodeoxycholic acid (GDCA), and deoxycholic acid (DCA) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).

Techniques: